Method for measuring refractive index of high melting point samples such as beeswax

    Beeswax, also known as yellow wax, beeswax. Beeswax is the substance secreted by the wax glands of the worker bees below the abdomen. Its main components are: acids, free fatty acids , free fatty alcohols and carbohydrates . In addition, there are carotenoids , vitamin A , aromatic substances and so on.

Beeswax has a wide range of uses in industrial and agricultural production. In the cosmetic manufacturing industry, many beauty products contain beeswax, such as bath lotions, lipsticks , and blushers. In the candle processing industry, beeswax can be used as the main raw material to manufacture various types of candles; in the pharmaceutical industry, beeswax can be used to manufacture Dental casting wax, base wax, stick wax, pill shell; can be used as food coating, packaging and outer clothing in the food industry; in agriculture and animal husbandry can be used to manufacture fruit trees and wood wax and pest adhesion agents; Beekeeping can produce nest bases and wax bowls.

GB /T 2431-2009 physical and chemical requirements of beeswax: melting point: 62-67 °C , refractive index (75 °C ): 1.4410-1.4430. Test procedure using Abbe refractometer:

The main requirements of this operation are two points: 1. The test temperature is 75 °C , and the temperature control device is required to control the temperature of the sample; 2. The operating temperature of the Abbe refractometer is that the prism must withstand a temperature of at least 75 degrees.

ATAGO's high temperature Abbe refractometer NAR-2T, with a maximum temperature of 120 °C , is ideally suited for the refractive index measurement of beeswax products. It is also used by Hangzhou Beehive Apiculture Co., Ltd. and Hangzhou Tianchu Honey Source Health Products Limited. Companies and other customers are used.

In addition, ATAGO's built-in Peltier temperature-controlled automatic digital tabletop refractometer Rx-5000i makes it easier and more accurate to test the refractive index of propolis.


Test steps:

1. Set temperature control to 75°C

2. Use pure water to adjust the zero point

3. Put the beeswax into the sample tank until the beeswax melts and the temperature stabilizes at 75°C.

4. Press the test key to read the value.

5. Repeat three times and average.

6. Clean the sample cell with warm water  
Beeswax slowly melts in the sample tank
7. Test the next sample.


Beeswax melts almost completely


 


Sample 1: Refractive index: 1.44066 Sample 2: Refractive index: 1.44070


Measuring the refractive index of beeswax with Peltier temperature-controlled automatic digital tabletop refractometer Rx-5000i, compared to the Abbe refractometer, has the following obvious advantages:


1. The Rx-5000i does not need to melt the propolis first and then transfer it to the prism; just place the propolis in the sample tank and the propolis will melt during the temperature control process.

2. The built-in Peltier temperature control is much faster than the water bath temperature control. The temperature is increased and the temperature is reduced. There is no temperature loss and the control is more accurate.

3. The Rx-5000i large LCD screen directly displays the measurement results. No need to adjust the outside line, check the scale line to read the result.

4. The measurement accuracy is higher, accurate to ±0.00003 RI.

The measurement of the refractive index of other high melting point samples other than beeswax can be performed with reference to this operation. For more product information, or for samples to be tested, please contact ATAGO China, we will be happy to serve you.

ATAGO (Audio) China Marketing Department (Xuan)

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